Rat TRF(Transferrin) ELISA Kit

SKU: ELK11067

Size:96T

Reactivity:Rat

Alternative Names:TF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin

Assay Type:Sandwich

Sensitivity:4.27 µg/mL

Standard:1000 µg/mL

Detection range:15.63-1000 µg/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Tumor immunity;Infection immunity;Immune molecule;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TRF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TRF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRF in the samples is then determined by comparing the OD of the samples to the standard curve.

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