Rat TCN2(Transcobalamin II, Macrocytic Anemia) ELISA Kit

SKU: ELK7374

Size:96T

Reactivity:Rat

Alternative Names:TC2; Transcobalamin II

Assay Type:Sandwich

Sensitivity:30 pg/mL

Standard:5000 pg/mL

Detection range:78.13-5000 pg/mL

Sample type:Serum, plasma, tissue homogenates and other biological fluids.

Assay length:3.5h

Research Area:Metabolic pathway;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TCN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TCN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TCN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TCN2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q9R0D6