Rat SCGN(Secretagogin) ELISA Kit

SKU: ELK6364

Size:96T

Reactivity:Rat

Alternative Names:SECRET; SEGN; CALBL; Calbindin-Like; Secretagogin,EF-Hand Calcium Binding Protein

Assay Type:Competitive Inhibition

Sensitivity:23.9 pg/mL

Standard:5000 pg/mL

Detection range:78.13-5000 pg/mL

Sample type:Serum, plasma and other biological fluids

Assay length:2h

Research Area:Metabolic pathway;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat SCGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SCGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SCGN in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q6R556