Rat Proliferating Cell Nuclear Antigen,PCNA ELISA KIT

SKU: E3412Ra

Size:96 Tests-1 Kit

Background:Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (By similarity). {ECO:0000250|UniProtKB:P12004}.

Research_area:

Applications:ELISA

Target_protein:Pcna

Reactivity:Rat

Assay type:Sandwich

Sensitivity:0.12ng/ml

Detection range:0.25-16ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P04961

MASS(Da):28749

GeneID:25737

Synonyms:Pcna

Gene names:Pcna

Standard solution:32ng/ml