Rat Phosphatidylinositol Binding Clathrin Assembly Protein,PICALM ELISA KIT

SKU: E2634Ra

Size:96 Tests-1 Kit

Background:Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF). {ECO:0000250|UniProtKB:Q13492, ECO:0000269|PubMed:11190274}.

Research_area:

Applications:ELISA

Target_protein:Picalm

Reactivity:Rat

Assay type:Sandwich

Sensitivity:0.016ng/ml

Detection range:0.05-10ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Phosphatidylinositol Binding Clathrin Assembly Protein

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:O55012

MASS(Da):69286

GeneID:89816

Synonyms:Picalm Calm

Gene names:Picalm

Standard solution:12.8ng/ml