Rat PDGF-AB ELISA Kit PicoKine®

SKU: EK0485

Size: 96 wells/kit, with removable strips.

Storage: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Form:

Reactivity: Rat

Applications: ELISA

Application Details:

Gene Name: PDGFB, PDGFA

Specificity: Natural and recombinant rat PDGF-AB

Background: The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant rat PDGF-AB (A chain Ser87-Arg196; B chain Ser74-Thr182) with the molecular mass of 24.8KDa.

Immunogen: Expression system for standard: E.coli; Immunogen sequence: (R86-K204) + (S74-T182)

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Reconstitution:

Reference:

Uniprot ID: Q05028/P28576

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Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

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Gene ID: 24628, 25266

Protein Name:

Gene Full Name: platelet-derived growth factor beta polypeptide,platelet-derived growth factor alpha polypeptide

Synonyms: PDGF A chain; PDGF B chain; PDGF1; PDGF2; PDGFA; PDGFB; Platelet derived growth factor AB heterodimer