Rat p-JNK(Phosphorylated c-Jun N-terminal kinases) ELISA Kit

SKU: ELK0960

Size:96T

Reactivity:Rat

Alternative Names:Bsk; BSK; CG5680; D-JNK; D-junk; DBSK/JNK; dJnk; dJNK; DJNK; DJNK/bsk; Dmel\CG5680; DmJNK; dNJNK; jnk; Jnk; JNK; jnk (bsk); JNK/SAPK; Junk; p-JNK; P-JNK; pJNK; SAPKa

Assay Type:Sandwich

Sensitivity:0.148 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:Enzyme & Kinase;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-JNK in the samples is then determined by comparing the OD of the samples to the standard curve.

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