Rat MMP-2 PicoKine® Quick ELISA Kit

SKU: FEK0639

Size: 96 wells/kit, with removable strips.

Storage: Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Form:

Reactivity: Rat

Applications: ELISA

Application Details:

Gene Name: CST3

Specificity: Natural and recombinant Rat Mmp2

Background: Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

Immunogen: Expression system for standard: NS0; Immunogen sequence: A30-C662

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Reference:

Uniprot ID: P33436

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Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

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Gene ID: 81686

Protein Name: 72 kDa type IV collagenase

Gene Full Name: matrix metallopeptidase 2

Synonyms: 72 kDa gelatinase; 72 kDa type IV collagenase; CLG4; CLG4A; Gelatinase A; Matrix metalloproteinase 2; MMP 2; MMP II; MMP2; MMP-2; MONA; TBE 1