Rat MGP(Matrix Gla Protein) ELISA Kit

SKU: ELK6005

Size:96T

Reactivity:Rat

Alternative Names:MGLAP; GIG36; Cell growth-inhibiting gene 36 protein

Assay Type:Sandwich

Sensitivity:0.28 ng/mL

Standard:50 ng/mL

Detection range:0.79-50 ng/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Metabolic pathway;Hematology;Bone metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MGP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MGP in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P08494