Rat KLK1(Kallikrein 1) ELISA Kit

SKU: ELK10629

Size:96T

Reactivity:Rat

Alternative Names:KLKR; Klk6; HK1; Kallikrein 1,Renal/Pancreas/Salivary; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein

Assay Type:Competitive Inhibition

Sensitivity:0.11 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:Serum, plasma, other biological fluids

Assay length:2h

Research Area:Enzyme & Kinase;Tumor immunity;Hepatology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat KLK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P00758