Rat Kelch-like Ech-associated Protein 1, KEAP1 ELISA Kit

SKU: E2124Ra

Size:96 Tests-1 Kit

Background:Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination. KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2. The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation (By similarity). The BCR(KEAP1) complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome (By similarity).

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:KEAP1

Reactivity:Rat

Assay type:Sandwich

Sensitivity:9.16ng/L

Detection range:20-6000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Kelch-like ECH-associated protein 1

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P57790

MASS(Da):69,399

GeneID:117519

Synonyms:Cytosolic inhibitor of Nrf2;Inrf2;KEAP1;Kelch-like ECH-associated protein 1

Gene names:Keap1

Standard solution:6400ng/L