Rat HbA1c(Glycated Hemoglobin A1c) ELISA Kit

SKU: ELK0120

Size:96T

Reactivity:Rat

Alternative Names:Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

Assay Type:Sandwich

Sensitivity:4.25 µg/mL

Standard:1000 µg/mL

Detection range:15.63-1000 µg/mL

Sample type:plasma and erythrocyte lysates

Assay length:3.5h

Research Area:Diagnosis of diabetes mellitus

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HbA1c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HbA1c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.

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