Rat Cytochrome P450 7A1,CYP7A1 ELISA KIT

SKU: E2352Ra

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols) (PubMed:1694852, PubMed:2335522). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:1694852, PubMed:2335522). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis (PubMed:1694852, PubMed:2335522). 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives (By similarity). {ECO:0000250|UniProtKB:P22680, ECO:0000269|PubMed:1694852, ECO:0000269|PubMed:2335522}.

Research_area:

Applications:ELISA

Target_protein:Cyp7a1

Reactivity:Rat

Assay type:Sandwich

Sensitivity:0.13ng/ml

Detection range:0.3-90ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name: Cholesterol 7-alpha-monooxygenase

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P18125

MASS(Da):56883

GeneID:25428

Synonyms:Cyp7a1 Cyp7

Gene names:Cyp7a1

Standard solution:96ng/ml