Rat Adenylyl cyclase 1 (AC-1) ELISA Kit

SKU: AE23273RA

Size: 96T. Other sizes are also available.

Species Reactivity: Rat (Rattus norvegicus)

UniProt: Q05922

Abbreviation: AC-1

Alternative Names: AC1; 3 ;5 -cyclic AMP synthetase|ATP pyrophosphate-lyase|Ca(2+)/calmodulin-activated adenylyl cyclase|adenyl cyclase|adenylate cyclase 1|adenylate cyclase type I

Application: ELISA

Range: 31.25-2000 pg/mL

Sensitivity: 7.81 pg/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate AC-1 in samples. An antibody specific for AC-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAC-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AC-1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AC-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).