Rabbit HP(Haptoglobin) ELISA Kit

SKU: ELK11140

Size:96T

Reactivity:Rabbit

Alternative Names:HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin; Hpt; Haptoglobin

Assay Type:Sandwich

Sensitivity:0.69 µg/mL

Standard:100 µg/mL

Detection range:1.56-100 µg/mL

Sample type:Serum, plasma and other biological fluids.

Assay length:3.5h

Research Area:Infection immunity;Hematology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit HP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit HP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit HP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit HP in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P19007