PXN (Ab-31) Antibody - Cat. #: CSB-PA944033

SKU: CSB-PA944033

Size :100ul

Clone Number:

Aliases:FLJ16691 antibody; FLJ23042 antibody; Paired box protein Pax 1 antibody; PAX 1 antibody; PAX1 antibody; PAXI_HUMAN antibody; Paxillin alpha antibody; Paxillin antibody; PXN antibody; PXN protein antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:P49023

Immunogen:Peptide sequence around aa.29~33 (T-P-Y-S-Y) derived from Human Paxillin.

Raised in:Rabbit

Species Reactivity:Human

Tested Applications:ELISA, WB, IHC; WB:1:500-1:1000, IHC:1:50-1:200

Background:

An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

Davidson D, et al. (2001) EMBO J; 20(13): 3414-3426
Kook S, et al. (2000) Mol Biol Cell; 11(3): 929-939
Fleming I, et al. (1999) Proc Natl Acad Sci U S A; 96(3): 1123-1128
Goldberg MB, et al. (2001) Microbiol Mol Biol Rev; 65(4): 595-626
Thomas JT, et al. (1999) Proc Natl Acad Sci U S A; 96(15): 8449-8454

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Conjugate:Non-conjugated

Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:PXN

Research Areas:Cancer;Signal transduction