Purified Rabbit anti-Wilms Tumor 1 Antibody

SKU: DL97185A

Size 1: 100 ul

Synonyms : Wilms tumor protein; WT33

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Wilms Tumor 1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Wilms Tumor 1

WB description: Western blot analysis of Wilms Tumor 1 expression in A549 (A), MCF7 (B), mouse heart (C) whole cell lysates.

IHC description: Immunohistochemical analysis of Wilms Tumor 1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Wilms Tumor 1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 7490

Gene ID (Mouse):

Gene ID(Rat): 24883

SwissProt (Human): P19544

SwissProt (Mouse): P22561

SwissProt (Rat): P49952

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.