Purified Rabbit anti-TRIM69 Antibody

SKU: DL97990A

Size 1: 100 ul

Synonyms : RNF36; E3 ubiquitin-protein ligase TRIM69; RFP-like domain-containing protein trimless; RING finger protein 36; Tripartite motif-containing protein 69

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to TRIM69

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human TRIM69

WB description: Western blot analysis of TRIM69 expression in mouse liver (A) whole cell lysates.

IHC description: Immunohistochemical analysis of TRIM69 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of TRIM69 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 140691

Gene ID (Mouse): 70928

Gene ID(Rat): 311373

SwissProt (Human): Q86WT6

SwissProt (Mouse): Q80X56

SwissProt (Rat): Q5BK82

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.