Purified Rabbit anti-SMAD3 (pS204) Antibody

SKU: DL90708A

Size 1: 100 ul

Synonyms : MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, C, Mk, P, S

Applications: WB, IF/IC, IP

Background: Rabbit polyclonal antibody to SMAD3 (pS204)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMAD3. The exact sequence is proprietary.

WB description: Western blot analysis of SMAD3 (pS204) expression in HeLa UV-treated (A), SP2/0 UV-treated (B), H9C2 UV-treated (C) whole cell lysates.

IHC description:

IF/ICC description: Immunofluorescent analysis of SMAD3 (pS204) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 4088

Gene ID (Mouse): 17127

Gene ID(Rat): 25631

SwissProt (Human): P84022

SwissProt (Mouse): Q8BUN5

SwissProt (Rat): P84025

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.