Purified Rabbit anti-SMAD2 (pS467) Antibody

SKU: DL93243A

Size 1: 100 ul

Synonyms : MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, Mk, P, Rb, S, Z

Applications: ELISA, WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to SMAD2 (pS467)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SMAD2 (pS467). The exact sequence is proprietary.

WB description: Western blot analysis of SP1 (pT453) expression in Jurkat (A), mouse melanoma cancer (B) whole cell lysates.

IHC description: Immunohistochemical analysis of SP1 (pT453) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of SP1 (pT453) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), ChIP (Use at an assay dependent concentration)

Gene ID(Human): 6667

Gene ID (Mouse): 20683

Gene ID(Rat): 24790

SwissProt (Human): P08047

SwissProt (Mouse): O89090

SwissProt (Rat): Q01714

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.