Purified Rabbit anti-SHCA (pY349) Antibody

SKU: DL93234A

Size 1: 100 ul

Synonyms : SHC; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B

Applications: WB, IHC

Background: Rabbit polyclonal antibody to SHCA (pY349)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SHCA (pY349). The exact sequence is proprietary.

WB description: Western blot analysis of SHCA (pY349) expression in HeLa EGF-treated (A), HuvEc EGF-treated (B) whole cell lysates.

IHC description: Immunohistochemical analysis of SHCA (pY349) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 6464

Gene ID (Mouse): 20416

Gene ID(Rat): 85385

SwissProt (Human): P29353

SwissProt (Mouse): P98083

SwissProt (Rat): Q5M824

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.