Purified Rabbit anti-RRM2 Antibody

SKU: DL96041A

Size 1: 100 ul

Synonyms : RR2; Ribonucleoside-diphosphate reductase subunit M2; Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to RRM2

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human RRM2

WB description: Western blot analysis of RRM2 expression in MCF7 (A), Hela (B), Jurakt (C), mouse thymus (D) whole cell lysates.

IHC description: Immunohistochemical analysis of RRM2 staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of RRM2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/10 - 1/100)

Gene ID(Human): 6241

Gene ID (Mouse): 20135

Gene ID(Rat): 362720

SwissProt (Human): P31350

SwissProt (Mouse): P11157

SwissProt (Rat): Q4KLN6

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.