Purified Rabbit anti-RAD9A Antibody

SKU: DL97631A

Size 1: 100 ul

Synonyms : RAD9A; Cell cycle checkpoint control protein RAD9A; hRAD9; DNA repair exonuclease rad9 homolog A

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to RAD9A

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human RAD9A

WB description: Western blot analysis of RAD9A expression in mouse testis (A) whole cell lysates.

IHC description: Immunohistochemical analysis of RAD9A staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of RAD9A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 5883

Gene ID (Mouse): 19367

Gene ID(Rat):

SwissProt (Human): Q99638

SwissProt (Mouse): Q9Z0F6

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.