Purified Rabbit anti-RAD21 Antibody

SKU: DL93988A

Size 1: 100 ul

Synonyms : HR21; KIAA0078; NXP1; Double-strand-break repair protein rad21 homolog; hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, Z

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to RAD21

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RAD21. The exact sequence is proprietary.

WB description: Western blot analysis of RAD21 expression in Jurkat (A), A431 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of RAD21 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of RAD21 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 5885

Gene ID (Mouse): 19357

Gene ID(Rat):

SwissProt (Human): O60216

SwissProt (Mouse): Q61550

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.