Purified Rabbit anti-PRPF3 Antibody

SKU: DL96083A

Size 1: 100 ul

Synonyms : HPRP3; PRP3; U4/U6 small nuclear ribonucleoprotein Prp3; Pre-mRNA-splicing factor 3; hPrp3; U4/U6 snRNP 90 kDa protein

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to PRPF3

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human PRPF3

WB description: Western blot analysis of PRPF3 expression in A549 (A), HepG2 (B), THP1 (C), mouse spleen (D), mouse testis (E) whole cell lysates.

IHC description: Immunohistochemical analysis of PRPF3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of PRPF3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 9129

Gene ID (Mouse): 70767

Gene ID(Rat):

SwissProt (Human): O43395

SwissProt (Mouse): Q922U1

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.