Purified Rabbit anti-PRKAR1B Antibody

SKU: DL90932A

Size 1: 100 ul

Synonyms : cAMP-dependent protein kinase type I-beta regulatory subunit

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, C, D, Z

Applications: WB, IHC

Background: Rabbit polyclonal antibody to PRKAR1B

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PRKAR1B. The exact sequence is proprietary.

WB description: Western blot analysis of PRKAR1B expression in MCF7 (A), KNRK (B), NIH3T3 (C), HeLa (D), BT20 (E) whole cell lysates.

IHC description: Immunohistochemical analysis of PRKAR1B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 5575

Gene ID (Mouse): 19085

Gene ID(Rat):

SwissProt (Human): P31321

SwissProt (Mouse): P12849

SwissProt (Rat): P81377

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.