Purified Rabbit anti-PP1 beta Antibody

SKU: DL95889A

Size 1: 100 ul

Synonyms : Serine/threonine-protein phosphatase PP1-beta catalytic subunit; PP-1B; PPP1CD

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to PP1 beta

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human PP1 beta

WB description: Western blot analysis of PP1 beta expression in HL60 (A), PC3 (B), Hela (C), mouse heart (D) whole cell lysates.

IHC description: Immunohistochemical analysis of PP1 beta staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of PP1 beta staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 5500

Gene ID (Mouse): 19046

Gene ID(Rat): 25594

SwissProt (Human): P62140

SwissProt (Mouse): P62141

SwissProt (Rat): P62142

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.