Purified Rabbit anti-PKC beta (pS661) Antibody

SKU: DL93785A

Size 1: 100 ul

Synonyms : PKCB; PRKCB1; Protein kinase C beta type; PKC-B; PKC-beta

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, Mk, P, Rb

Applications: WB, IHC

Background: Rabbit polyclonal antibody to PKC beta (pS661)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PKC beta. The exact sequence is proprietary.

WB description: Western blot analysis of PKC beta (pS661) expression in K562 PMA-treated (A), HeLa (B) whole cell lysates.

IHC description: Immunohistochemical analysis of PKC beta (pS661) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 5579

Gene ID (Mouse): 18751

Gene ID(Rat): 25023

SwissProt (Human): P05771

SwissProt (Mouse): P68404

SwissProt (Rat): P68403

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.