Purified Rabbit anti-PKA C alpha/beta Antibody

SKU: DL90927A

Size 1: 100 ul

Synonyms : PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACB; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, D, P, S

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to PKA C alpha/beta

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PKA C alpha/beta. The exact sequence is proprietary.

WB description: Western blot analysis of PKA C alpha/beta expression in HEK293T (A), Raw264.7 (B), PC12 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of PKA C alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of PKA C alpha/beta staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 5566; 5567

Gene ID (Mouse): 18747; 18749

Gene ID(Rat): 293508

SwissProt (Human): P17612; P22694

SwissProt (Mouse): P05132; P68181

SwissProt (Rat): P27791; P68182

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.