Purified Rabbit anti-p67 phox Antibody

SKU: DL95899A

Size 1: 100 ul

Synonyms : NOXA2; P67PHOX; Neutrophil cytosol factor 2; NCF-2; 67 kDa neutrophil oxidase factor; NADPH oxidase activator 2; Neutrophil NADPH oxidase factor 2; p67-phox

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to p67 phox

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human p67 phox

WB description: Western blot analysis of p67 phox expression in THP1 (A), mouse spleen (B) whole cell lysates.

IHC description: Immunohistochemical analysis of p67 phox staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of p67 phox staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/20 - 1/100)

Gene ID(Human): 4688

Gene ID (Mouse): 17970

Gene ID(Rat): 364018

SwissProt (Human): P19878

SwissProt (Mouse): O70145

SwissProt (Rat): A7E3N2

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.