Purified Rabbit anti-Nucleolin Antibody

SKU: DL96959A

Size 1: 100 ul

Synonyms : Nucleolin; Protein C23

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Nucleolin

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Nucleolin

WB description: Western blot analysis of Nucleolin expression in MCF7 (A), A549 (B), THP1 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of Nucleolin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200)

Gene ID(Human): 4691

Gene ID (Mouse): 17975

Gene ID(Rat):

SwissProt (Human): P19338

SwissProt (Mouse): P09405

SwissProt (Rat): P13383

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.