Purified Rabbit anti-MRPL15 Antibody

SKU: DL91637A

Size 1: 100 ul

Synonyms : 39S ribosomal protein L15, mitochondrial; L15mt; MRP-L15

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, Mk

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to MRPL15

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MRPL15. The exact sequence is proprietary.

WB description: Western blot analysis of MRPL15 expression in PANC1 (A), HuvEc (B) whole cell lysates.

IHC description: Immunohistochemical analysis of MRPL15 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of MRPL15 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 29088

Gene ID (Mouse): 27395

Gene ID(Rat):

SwissProt (Human): Q9P015

SwissProt (Mouse): Q9CPR5

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.