Purified Rabbit anti-mPR gamma Antibody

SKU: DL94854A

Size 1: 100 ul

Synonyms : MPRG; Membrane progestin receptor gamma; mPR gamma; Progestin and adipoQ receptor family member 5; Progestin and adipoQ receptor family member V

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, S

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to mPR gamma

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human mPR gamma. The exact sequence is proprietary.

WB description: Western blot analysis of mPR gamma expression in HuvEc (A) whole cell lysates.

IHC description: Immunohistochemical analysis of mPR gamma staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of mPR gamma staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 54852

Gene ID (Mouse): 74090

Gene ID(Rat):

SwissProt (Human): Q9NXK6

SwissProt (Mouse): Q9DCU0

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.