Purified Rabbit anti-MKK1/2 (pS218/222) Antibody

SKU: DL92489A

Size 1: 100 ul

Synonyms : MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, C, D, Rb, Z

Applications: WB, IHC

Background: Rabbit polyclonal antibody to MKK1/2 (pS218/222)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MKK1/2 (pS218/222). The exact sequence is proprietary.

WB description: Western blot analysis of MKK1/2 (pS218/222) expression in HeLa EGF-treated (A), A431 EGF-treated (B) whole cell lysates.

IHC description: Immunohistochemical analysis of MKK1/2 (pS218/222) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 5604; 5605

Gene ID (Mouse): 26395; 26396

Gene ID(Rat): 170851; 58960

SwissProt (Human): Q02750; P36507

SwissProt (Mouse): P31938; Q63932

SwissProt (Rat): Q01986; P36506

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.