Purified Rabbit anti-MeCP2 Antibody

SKU: DL96114A

Size 1: 100 ul

Synonyms : Methyl-CpG-binding protein 2; MeCp-2 protein; MeCp2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to MeCP2

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide of human MeCP2

WB description: Western blot analysis of MeCP2 expression in HT29 (A), Jurkat (B), mouse spleen (C), mouse lung (D) whole cell lysates.

IHC description: Immunohistochemical analysis of MeCP2 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of MeCP2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 4204

Gene ID (Mouse): 17257

Gene ID(Rat): 29386

SwissProt (Human): P51608

SwissProt (Mouse): Q9Z2D6

SwissProt (Rat): Q00566

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.