Purified Rabbit anti-LYPLA1 Antibody

SKU: DL92382A

Size 1: 100 ul

Synonyms : APT1; LPL1; Acyl-protein thioesterase 1; APT-1; hAPT1; Lysophospholipase 1; Lysophospholipase I; LPL-I; LysoPLA I

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, Rb

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to LYPLA1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LYPLA1. The exact sequence is proprietary.

WB description: Western blot analysis of LYPLA1 expression in MDAMB453 (A), mouse kidney (B), rat kidney (C) whole cell lysates.

IHC description: Immunohistochemical analysis of LYPLA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of LYPLA1 staining in MDAMB453 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 10434

Gene ID (Mouse): 18777

Gene ID(Rat): 25514

SwissProt (Human): O75608

SwissProt (Mouse): P97823

SwissProt (Rat): P70470

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.