Purified Rabbit anti-Lamin B2 Antibody

SKU: DL96258A

Size 1: 100 ul

Synonyms : LMN2; Lamin-B2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Lamin B2

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Lamin B2

WB description: Western blot analysis of Lamin B2 expression in HepG2 (A), MCF7 (B), mouse liver (C), mouse heart (D) whole cell lysates.

IHC description: Immunohistochemical analysis of Lamin B2 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Lamin B2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 84823

Gene ID (Mouse): 16907

Gene ID(Rat):

SwissProt (Human): Q03252

SwissProt (Mouse): P21619

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.