Purified Rabbit anti-Kir6.2 (pT224) Antibody

SKU: DL94755A

Size 1: 100 ul

Synonyms : ATP-sensitive inward rectifier potassium channel 11; IKATP; Inward rectifier K(+) channel Kir6.2; Potassium channel inwardly rectifying subfamily J member 11

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, P, Rb

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Kir6.2 (pT224)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Kir6.2. The exact sequence is proprietary.

WB description: Western blot analysis of Kir6.2 (pT224) expression in MCF7 (A), mouse liver (B), rat liver (C) whole cell lysates.

IHC description: Immunohistochemical analysis of Kir6.2 (pT224) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Kir6.2 (pT224) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/100 - 1/500)

Gene ID(Human): 3767

Gene ID (Mouse): 16514

Gene ID(Rat): 83535

SwissProt (Human): Q14654

SwissProt (Mouse): Q61743

SwissProt (Rat): P70673

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.