Purified Rabbit anti-Kir5.1 (pS416) Antibody

SKU: DL99236A

Size 1: 100 ul

Synonyms : KCNJ16; Inward rectifier potassium channel 16; Inward rectifier K(+) channel Kir5.1; Potassium channel inwardly rectifying subfamily J member 16

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IH, IF/IC

Background: Rabbit polyclonal antibody to Kir5.1 (pS416)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Kir5.1 with a phosphorylation site at S416. The exact sequence is proprietary.

WB description: Western blot analysis of Kir5.1 (pS416) expression in HEK293T (A), HuT78 (B), mouse spleen (C), rat spleen (D) whole cell lysates.

IHC description: Immunohistochemical analysis of Kir5.1 (pS416) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Kir5.1 (pS416) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 3773

Gene ID (Mouse): 16517

Gene ID(Rat):

SwissProt (Human): Q9NPI9

SwissProt (Mouse): Q9Z307

SwissProt (Rat): P52191

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.