Purified Rabbit anti-IKK beta (pY199) Antibody

SKU: DL90585A

Size 1: 100 ul

Synonyms : IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, P

Applications: WB, IHC, IP

Background: Rabbit polyclonal antibody to IKK beta (pY199)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IKK beta. The exact sequence is proprietary.

WB description: Western blot analysis of IKK beta (pY199) expression in HeLa LPS-treated (A), Raw264.7 LPS-treated (B), PC12 LPS-treated (C) whole cell lysates.

IHC description: Immunohistochemical analysis of IKK beta (pY199) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Gene ID(Human): 3551

Gene ID (Mouse): 16150

Gene ID(Rat): 84351

SwissProt (Human): O14920

SwissProt (Mouse): O88351

SwissProt (Rat): Q9QY78

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.