Purified Rabbit anti-HSP27 (pS82) Antibody

SKU: DL94958A

Size 1: 100 ul

Synonyms : HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, B, D

Applications: WB, IHC

Background: Rabbit polyclonal antibody to HSP27 (pS82)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSP27 (pS82). The exact sequence is proprietary.

WB description: Western blot analysis of HSP27 (pS82) expression in A549 (A), HepG2 (B), K562 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of HSP27 (pS82) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 3315

Gene ID (Mouse): 15507

Gene ID(Rat):

SwissProt (Human): P04792

SwissProt (Mouse): P14602

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.