Purified Rabbit anti-HSD17B2 Antibody

SKU: DL96873A

Size 1: 100 ul

Synonyms : EDH17B2; Estradiol 17-beta-dehydrogenase 2; 17-beta-hydroxysteroid dehydrogenase type 2; 17-beta-HSD 2; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E2DH; Microsomal 17-beta-hydroxysteroid dehydrogenase; Testosterone 17-beta-dehydrogenase

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to HSD17B2

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human HSD17B2

WB description: Western blot analysis of HSD17B2 expression in SW480 (A), mouse liver (B), mouse kidney (C) whole cell lysates.

IHC description: Immunohistochemical analysis of HSD17B2 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of HSD17B2 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 3294

Gene ID (Mouse): 15486

Gene ID(Rat): 79243

SwissProt (Human): P37059

SwissProt (Mouse): P51658

SwissProt (Rat): Q62730

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.