Purified Rabbit anti-HRP2 Antibody

SKU: DL96473A

Size 1: 100 ul

Synonyms : HDGF2; Hepatoma-derived growth factor-related protein 2; HRP-2; Hepatoma-derived growth factor 2; HDGF-2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to HRP2

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human HRP2

WB description: Western blot analysis of HRP2 expression in HepG2 (A), Hela (B) whole cell lysates.

IHC description: Immunohistochemical analysis of HRP2 staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of HRP2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 84717

Gene ID (Mouse): 15193

Gene ID(Rat):

SwissProt (Human): Q7Z4V5

SwissProt (Mouse): Q3UMU9

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.