Purified Rabbit anti-HNMT Antibody

SKU: DL96005A

Size 1: 100 ul

Synonyms : Histamine N-methyltransferase; HMT

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to HNMT

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human HNMT

WB description: Western blot analysis of HNMT expression in HepG2 (A), Hela (B), mouse liver (C), mouse brain (D) whole cell lysates.

IHC description: Immunohistochemical analysis of HNMT staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of HNMT staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 3176

Gene ID (Mouse): 140483

Gene ID(Rat): 81676

SwissProt (Human): P50135

SwissProt (Mouse): Q91VF2

SwissProt (Rat): Q01984

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.