Purified Rabbit anti-Histone H2A.Z (AcK5) Antibody

SKU: DL93667A

Size 1: 100 ul

Synonyms : H2AZ; Histone H2A.Z; H2A/z

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, D, Mk, P, S, Z

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Histone H2A.Z (AcK5)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H2A.Z. The exact sequence is proprietary.

WB description: Western blot analysis of Histone H2A.Z (AcK5) expression in K562 TSA-treated (A), HeLa TSA-treated (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Histone H2A.Z (AcK5) staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Histone H2A.Z (AcK5) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 3015

Gene ID (Mouse): 51788

Gene ID(Rat): 58940

SwissProt (Human): P0C0S5

SwissProt (Mouse): P0C0S6

SwissProt (Rat): P0C0S7

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.