Purified Rabbit anti-FIP1L1 Antibody

SKU: DL96407A

Size 1: 100 ul

Synonyms : FIP1; RHE; Pre-mRNA 3'-end-processing factor FIP1; hFip1; FIP1-like 1 protein; Factor interacting with PAP; Rearranged in hypereosinophilia

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to FIP1L1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human FIP1L1

WB description: Western blot analysis of FIP1L1 expression in Jurkat (A), MCF7 (B), mouse thymus (C), rat lung (D) whole cell lysates.

IHC description: Immunohistochemical analysis of FIP1L1 staining in mouse liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of FIP1L1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 81608

Gene ID (Mouse): 66899

Gene ID(Rat): 289582

SwissProt (Human): Q6UN15

SwissProt (Mouse): Q9D824

SwissProt (Rat): Q5U317

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.