Purified Rabbit anti-EPS8L2 Antibody

SKU: DL91762A

Size 1: 100 ul

Synonyms : EPS8R2; Epidermal growth factor receptor kinase substrate 8-like protein 2; EPS8-like protein 2; Epidermal growth factor receptor pathway substrate 8-related protein 2; EPS8-related protein 2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to EPS8L2

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EPS8L2. The exact sequence is proprietary.

WB description: Western blot analysis of EPS8L2 expression in HepG2 (A), HeLa (B), A431 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of EPS8L2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of EPS8L2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 64787

Gene ID (Mouse): 98845

Gene ID(Rat):

SwissProt (Human): Q9H6S3

SwissProt (Mouse): Q99K30

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.