Purified Rabbit anti-EPHA2 (pY588/596) Antibody

SKU: DL93632A

Size 1: 100 ul

Synonyms : EPHA2; ECK; Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; EPHA3; ETK; ETK1; HEK; TYRO4; Ephrin type-A receptor 3; EPH-like kinase 4; EK4; hEK4; HEK; Human embryo kinase; Tyrosine-protein kinase TYRO4; Tyrosine-protein kinase receptor ETK1; Eph-like tyrosine kinase 1; EPHA4; HEK8; SEK; TYRO1; Ephrin type-A receptor 4; EPH-like kinase 8; EK8; hEK8; Tyrosine-protein kinase TYRO1; Tyrosine-protein kinase receptor SEK

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, C, Mk

Applications: WB, IF/IC

Background: Rabbit polyclonal antibody to EPHA2 (pY588/596)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EPHA2. The exact sequence is proprietary.

WB description: Western blot analysis of EPHA2 (pY588/596) expression in A431 (A), mouse brain (B) whole cell lysates.

IHC description:

IF/ICC description: Immunofluorescent analysis of EPHA2 (pY588/596) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Gene ID(Human): 1969; 2042; 2043

Gene ID (Mouse): 13836; 13838

Gene ID(Rat): 29210

SwissProt (Human): P29317; P29320; P54764

SwissProt (Mouse): Q03145; P29319; Q03137

SwissProt (Rat): O08680

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.