Purified Rabbit anti-EGFR (pY1197) Antibody

SKU: DL95201A

Size 1: 100 ul

Synonyms : ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC

Background: Rabbit polyclonal antibody to EGFR (pY1197)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EGFR (pY1197). The exact sequence is proprietary.

WB description: Western blot analysis of EGFR (pY1197) expression in A431 (A), NIH3T3 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of EGFR (pY1197) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 1956

Gene ID (Mouse): 13649

Gene ID(Rat):

SwissProt (Human): P00533

SwissProt (Mouse): Q01279

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.