Purified Rabbit anti-E2F6 Antibody

SKU: DL90361A

Size 1: 100 ul

Synonyms : Transcription factor E2F6; E2F-6

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, P

Applications: WB, IHC, ChIP

Background: Rabbit polyclonal antibody to E2F6

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human E2F6. The exact sequence is proprietary.

WB description: Western blot analysis of E2F6 expression in HeLa (A), HepG2 (B), K562 (C), mouse muscle (D), rat heart (E) whole cell lysates.

IHC description: Immunohistochemical analysis of E2F6 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description: ChIP analysis of Mouse ES cell nuclear lysate, incubated for 16 hours at 4°C. Cross-linking (X-ChIP) using formaldehyde for 10 minutes. Positive control: Tuba3. Negative Control: Beta-Actin. Detection step: Real-time PCR.

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), ChIP (1/100 - 1/500)

Gene ID(Human): 1876

Gene ID (Mouse): 50496

Gene ID(Rat):

SwissProt (Human): O75461

SwissProt (Mouse): O54917

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.